Sadikshya Rijal

Hi, there!

I'm Sadikshya, and I like to build at the intersection of biology and engineering.

I'm a PhD candidate in Biological Design at Arizona State University, where I work on synthetic gene circuits, CRISPR interference, and biomolecular condensates. Outside the lab, I help run Nucleate Arizona and enjoy mentoring early-career scientists.

Latest Work

CRISPRi-linked multimodule negative feedback loops to address winner-take-all resource competition

Integrated dCas9 into the Escherichia coli genome to implement CRISPRi across two gene modules, mitigating Winner-Takes-All (WTA) resource competition and improving synthetic gene circuit predictability. Published in ACS Synthetic Biology (2025).

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Phase Separation to Resolve Growth-Related Circuit Failures

Engineering biomolecular condensates as a strategy to stabilize synthetic gene circuits under growth feedback. Published in Cell (2025).

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Evolutionary history of the DNA repair protein, Ku, in eukaryotes and prokaryotes

A phylogenetic study of the DNA repair protein Ku across eukaryotes and prokaryotes, using sequence data from NCBI and OrthoDB. Published in PLOS ONE (2025).

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Context-dependent redesign of robust synthetic gene circuits

An overview of how emerging circuit–host interactions, such as growth feedback and resource competition, impact both deterministic and stochastic circuit behaviors, and their control strategies. Published in Trends in Biotechnology (2025).

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Enhancing circuit stability under growth feedback with supplementary repressive regulation

Implementation of a repressive node to stabilize protein levels and increase the robustness of a bistable circuit in response to growth feedback. Published in Nucleic Acid Research (2025).

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Structure and Function of Major SARS-CoV-2 and SARS-CoV Proteins

An overview on structures and functions of major SARS-CoV and SARS-CoV-2 proteins. Published in Bioinformatics and Biology Insights (2025).

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Molecular identification of Coffea arabica varieties in Nepal: insights from phylogenetic analysis

Phylogenetic analysis of 25 coffee varieties in collaboration with the Coffee Development Center (CDC, Nepal); identified samples as Coffea arabica with 4.3% evolutionary divergence. Published in Journal of Agriculture and Environment (2025).

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