Latest Work
Publication
CRISPRi-linked multimodule negative feedback loops to address winner-take-all resource competition
Integrated dCas9 into the Escherichia coli genome to implement CRISPRi across two gene modules, mitigating Winner-Takes-All (WTA) resource competition and improving synthetic gene circuit predictability. Published in ACS Synthetic Biology (2025).
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Publication
Phase Separation to Resolve Growth-Related Circuit Failures
Engineering biomolecular condensates as a strategy to stabilize synthetic gene circuits under growth feedback. Published in Cell (2025).
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Publication
Evolutionary history of the DNA repair protein, Ku, in eukaryotes and prokaryotes
A phylogenetic study of the DNA repair protein Ku across eukaryotes and prokaryotes, using sequence data from NCBI and OrthoDB. Published in PLOS ONE (2025).
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Publication
Context-dependent redesign of robust synthetic gene circuits
An overview of how emerging circuit–host interactions, such as growth feedback and resource competition, impact both deterministic and stochastic circuit behaviors, and their control strategies. Published in Trends in Biotechnology (2025).
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Publication
Enhancing circuit stability under growth feedback with supplementary repressive regulation
Implementation of a repressive node to stabilize protein levels and increase the robustness of a bistable circuit in response to growth feedback. Published in Nucleic Acid Research (2025).
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Publication
Structure and Function of Major SARS-CoV-2 and SARS-CoV Proteins
An overview on structures and functions of major SARS-CoV and SARS-CoV-2 proteins. Published in Bioinformatics and Biology Insights (2025).
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Publication
Molecular identification of Coffea arabica varieties in Nepal: insights from phylogenetic analysis
Phylogenetic analysis of 25 coffee varieties in collaboration with the Coffee Development Center (CDC, Nepal); identified samples as Coffea arabica with 4.3% evolutionary divergence. Published in Journal of Agriculture and Environment (2025).
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